Polymerase Chain Reaction Lab :: essays research papers

Title Polymerase Chain Reaction Simulation ProposeThe propose of this research laboratory was to understand how by running a gel electrophoresis on a batch of DNA we are able to see how many approximately cycles it has gone through.Methods Casting the Agarose Gel In this experiment .8% event was used. By using a 250ml flaskful the buffer solution was prepared. Using the equating to make enough solution for the entire lab class the equation had to be multiplied by four. The contents of this equation were added to the 250ml flask and swirled to evenly distribute it contents. Then a mark was dictated on the outside of the flask to indicate the level of the solution before heating. The flask opening had perafilm placed over it so that there was little to no evaporation. The solution was then placed in the microwave and heated. The solution was then heated for one min and swirled for evenly dissolved Agarose. The Agarose was then cooled, so that it was not to hot and the plate would c rack. nearly water was added to the solution because of there was some evaporation during heating. Once the gel had cooled, it was poured into the plate between the guard dams. The plate was filled about half charge up the comb arms. These dams are placed in the plate to prevent leaking. Then the gel was added and allowed to completely soiditify, which takes around 20mins. Preparing the Gel for Electrophoresis once the rubber dams have been removed (carefully), the comb was then removed. Then the buffer was made. The buffer was made by using the equation, but also multiplying it by four, for the three lab groups. Then the chambers around the gel plate is filled with the buffer, just enough buffer to cover the gel plate in a genuinely small amount. Then the dyes were loaded to there correct wells. Once the gels were added (carefully) the lid was placed on the plate and corpse was turned on. The system ran for about 10mins. (Hint the system is running when there are bubbles occur ring in the buffer solution.Once the gel had been run the exactly gel had been removed from the buffer, placed on tin plunder and moisten with a small amount of buffer solution. Then the gel had a DNA Instastain sheet placed on top of it. The sheet was placed on the gel firmly and a beackr and gel casting tray were placed on top of the gel.